Confocal laser endomicroscopy and endocytoscopy are endoscopic technologies that permit highresolution assessment of gastrointestinal mucosal histology at a cellular and subcellular level. The key to the confocal approach is the use of spatial filtering to eliminate outof. Fluorescence microscope refers to any microscope that uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image. The use of confocal microscopy in scientific research is a great support to perform the threedimensional study of the. Confocal laser scanning microscopy, fluorescence, confocality, z stack. Abstract confocal scanning fluorescence microscopy has become widely used in cell biology. The unit currently stands with a confocal microscope lsm 710 zeiss, as well as other fluorescence microscopes leica dmire2 and olympus ckx41 and a cell live imaging station from leica with temperature and co2 controllers. Principles of fluorescence techniques 2009 chicago, illinois. A plataforma especifica do software leica application suite las x facilita a configuracao dos experimentos mais complexos. Endomicroscopia laser confocal con sonda en pacientes con. Confocal microscopy offers several advantages over conventional optical microscopy, including controllable depth of field, the elimination of image degrading outoffocus information, and the ability to collect serial optical sections from thick specimens. A criticalcomponentinatwophotonmicroscopeisitslight figure 2 a schematic representation of the localization of twophoton excitation. Incubar 30 minutos a temperatura ambiente en c amara h umeda. Files are available under licenses specified on their description page.
Preservacao da proteina verde fluorescente no tecido osseo. Estos son, fluorescencia, luminiscencia y fosforescencia. Simples lupa e composto campo claro, ultravioleta, fluorescencia, campo escuro, polarizacao e. Por ello es imprescindible su limpieza, siguiendo las siguientes medidas. All structured data from the file and property namespaces is available under the creative commons cc0 license. Pdf microscopia confocal aplicada as ciencias biologicas. Fluorescence microscope refers to any microscope that uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence. A microscopia confocal e uma tecnica imagiologica desenvolvida primariamente por marvin minsky, em 1955. Scribd is the worlds largest social reading and publishing site. Thermo fisher scientific offers the highest quality fluorescence microscopy reagents and assays including cell structure and function dyes and stains, antifades and mountants, instrument calibration and setup tools and much more. The zeiss lsm 880 is a modern, flexible, confocal fluorescence microscope capable of looking at both fixed and live cells animals.
Fluorescence and confocal microscopy the university of. Principles of fluorescence techniques 2009 chicago. A criticalcomponentinatwophotonmicroscopeisitslight figure 2 a schematic representation of the localization of. Microscopia laser confocal, fluorescencia, confocalidad.
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